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ERX10441471: Sequel sequencing
1 PACBIO_SMRT (Sequel) run: 31,272 spots, 80.7M bases, 51.8Mb downloads

Submitted by: GENGS-CBMSO
Study: Leishmania infantum raw data of all the transcript isoforms generated with PacBio
show Abstracthide Abstract
Total RNA samples from three biological replicates (GC1003369, GC1003370, GC1003371) of Leishmania infantum (JPCM5 strain) promastigotes were separately retrotranscribed using poly-T oligonucleotide into cDNA. After a step of PCR amplification, the resulting DNA was sequenced using the Single Molecule, Real-Time (SMRT) Sequencing methodology (PacBio) by the sequencer: 1M V3.0 Long Read SMRTCell and chemistry. High quality reads were used to generate circular consensus sequences (CCS), which were classified as complete or truncated transcripts, depending on the presence at their 5'-end of the mini-exon (spliced-leader) sequence or not, respectively. Here, we are submitting both the raw data and the sequences of all the transcript isoforms generated in this study.
Sample: Leishmania infantum IsoSeq raw reads
SAMEA112765263 • ERS14766077 • All experiments • All runs
Library:
Instrument: Sequel
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Runs: 1 run, 31,272 spots, 80.7M bases, 51.8Mb
Run# of Spots# of BasesSizePublished
ERR1100482431,27280.7M51.8Mb2023-09-20

ID:
29654827

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